Bernard Levin, MD, David A. Lieberman, MD, FACG, Beth McFarland, MD, Robert A. Smith, PhD, Durado Brooks, MD, MPH, Kimberly S. Andrews, John Bond, MD, FACG, Chiranjeev Dash, MD, MPH, Francis M. Giardiello, MD, Seth Glick, MD, C. Daniel Johnson, MD, David A. Johnson, MD, FACG, Theodore R. Levin, MD, FACG, Perry J. Pickhardt, MD, Douglas K. Rex, MD, FACG, Alan Thorson, MD, Sidney J. Winawer, MD, MACG, for the American Cancer Society Colorectal Cancer Advisory Group, the US Multi-Society Task Force, and the American College of Radiology Colon Cancer Committee
CA Cancer J Clin 2008; 58:130–160; doi:10.3322/CA.2007.0018; published online 31 December 2008
This article is being published jointly in 2008 in CA: A Cancer Journal for Clinicians (online: March 5, 2008; print: May/June 2008), Gastroenterology (online: March 2008; print: May 2008), and Radiology (print: June 2008) by the American Cancer Society, the American Gastroenterological Association (AGA) Institute, and the Radiological Society of North America.
Published online through CA First Look at http://CAonline.AmCancer.Soc.org.
CME: To earn free CME credit for successfully completing the online quiz based on this article, go to http://CME.AmCancerSoc.org.
Copyright © 2008 American Cancer Society, Inc., and American Gastroenterological Association (AGA) Institute. Copying with attribution allowed for any noncommercial use of the work.
Dr. B. Levin is Professor Emeritus, The University of Texas MD Anderson Cancer Center, Houston, TX.
Dr. Lieberman is Chief, Division of Gastroenterology, Oregon Health and Science University, Portland Veterans Medical Center, Portland, OR.
Dr. McFarland is Adjunct Professor of Radiology, Washington University; St. Luke’s Hospital and Center for Diagnostic Imaging, Chesterfield, MO.
Dr. Smith is Director, Cancer Screening, Cancer Control Science Department, American Cancer Society, Atlanta, GA.
Dr. Brooks is Director, Prostate and Colorectal Cancer, Cancer Control Science Department, American Cancer Society, Atlanta, GA.
Ms. Andrews is Research Associate, Cancer Control Science Department, American Cancer Society, Atlanta, GA.
Dr. Dash is Doctoral Candidate, Department of Epidemiology, Emory University, Rollins School of Public Health, Atlanta, GA.
Dr. Giardiello is John G. Rangos Sr. Professor of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD.
Dr. Glick is Clinical Professor of Radiology, University of Pennsylvania Health System, Philadelphia, PA.
Dr. T. Levin is Staff Physician, Gastroenterology Department, Kaiser Permanente Walnut Creek Medical Center, Walnut Creek, CA.
Dr. Pickhardt is Associate Professor, Radiology Department, University of Wisconsin Hospital and Clinics, Madison, WI.
Dr. Rex is Professor of Medicine, Indiana University, Indianapolis, IN.
Dr. Thorson is Associate Professor of Surgery; and Program Director, Section of Colon and Rectal Surgery, Creighton University School of Medicine; and Clinical Associate Professor of Surgery, University of Nebraska College of Medicine, Omaha, NE.
Dr. Winawer is Attending Physician, Memorial Sloan-Kettering Cancer Center, New York, NY.
In the article "Screening and Surveillance for the Early Detection of Colorectal Cancer and Adenomatous Polyps, 2008: A Joint Guideline from the American Cancer Society, the US Multi-Society Task Force on Colorectal Cancer, and the American College of Radiology" (CA Cancer J Clin 2008, published online March 5, 2008), the names of 3 authors, John Bond, MD (Chief, Gastroenterology Section, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN), C. Daniel Johnson, MD (Chairman and Professor of Radiology, Radiology Department, Mayo Clinic, Scottsdale, AZ), and David A. Johnson, MD (Professor of Medicine, Chief of Gastroenterology, Eastern Virginia Medical School, Norfolk, VA), were inadvertently omitted. The author list should correctly read: Bernard Levin, MD; David A. Lieberman, MD; Beth McFarland, MD; Robert A. Smith, PhD; Durado Brooks,MD, MPH; Kimberly S. Andrews; John Bond, MD; Chiranjeev Dash, MD, MPH; Francis M. Giardiello, MD; Seth Glick, MD; C. Daniel Johnson, MD; David A. Johnson, MD; Theodore R. Levin, MD; Perry J. Pickhardt, MD; Douglas K. Rex, MD; Alan Thorson, MD; Sidney J. Winawer, MD; for the American Cancer Society Colorectal Cancer Advisory Group, the US Multi-Society Task Force, and the American College of Radiology Colon Cancer Committee.
In addition, the American Gastroenterological Association (AGA) Institute was incorrectly identified in the joint publication and copyright statements. The statements should read:
*This article is being published jointly in 2008 in CA: A Cancer Journal for Clinicians (online: March 5, 2008; print: May/June 2008), Gastroenterology (online: March 2008; print: May 2008), and Radiology (print: June 2008) by the American Cancer Society, the American Gastroenterological Association (AGA) Institute, and the Radiological Society of North America.
©2008 American Cancer Society, Inc., and American Gastroenterological Association (AGA) Institute. Copying with attribution allowed for any noncommercial use of the work.
The authors regret the errors.
In the United States, colorectal cancer (CRC) is the third most common cancer diagnosed among men and women and the second leading cause of death from cancer. CRC largely can be prevented by the detection and removal of adenomatous polyps, and survival is significantly better when CRC is diagnosed while still localized. In 2006 to 2007, the American Cancer Society, the US Multi Society Task Force on Colorectal Cancer, and the American College of Radiology came together to develop consensus guidelines for the detection of adenomatous polyps and CRC in asymptomatic average-risk adults. In this update of each organization’s guidelines, screening tests are grouped into those that primarily detect cancer early and those that can detect cancer early and also can detect adenomatous polyps, thus providing a greater potential for prevention through polypectomy. When possible, clinicians should make patients aware of the full range of screening options, but at a minimum they should be
prepared to offer patients a choice between a screening test that is effective at both early cancer detection and cancer prevention through the detection and removal of polyps and a screening test that primarily is effective at early cancer detection. It is the strong opinion of these 3 organizations that colon cancer prevention should be the primary goal of screening. (CA Cancer J Clin 2008;58:130–160.) © American Cancer Society, Inc., 2008.
In the United States, colorectal cancer (CRC) is the third most common cancer diagnosed in men and women and the second leading cause of death from cancer (1). In 2008, it is estimated that 148,810 men and women will be diagnosed with CRC and 49,960 will die from this disease (1). Five-year survival is 90% if the disease is diagnosed while still localized (ie, confined to the wall of the bowel), but only 68% for regional disease (ie, disease with lymph node involvement), and only 10% if distant metastases are present (2). Recent trends in CRC incidence and mortality reveal declining rates, which have been attributed to reduced exposure to risk factors, screening’s effect on early detection and prevention through polypectomy, and improved treatment (3). However, in the near term, even greater incidence and mortality reductions could be achieved if a greater proportion of adults received regular screening. Although prospective randomized trials and observational studies have demonstrated mortality reductions associated with early detection of invasive disease, as well as removal of adenomatous polyps (4–7), a majority of US adults are not receiving regular age- and risk-appropriate screening or have never been screened at all (8,9).
The goal of cancer screening is to reduce mortality through a reduction in incidence of advanced disease. To this end, modern CRC screening can achieve this goal through the detection of early-stage adenocarcinomas and the detection and removal of adenomatous polyps, the latter generally accepted as nonobligate precursor lesions. Adenomatous polyps are common in adults over age 50 years, but the majority of polyps will not develop into adenocarcinoma; histology and size determine their clinical importance (10,11). The most common and clinically important polyps are adenomatous polyps, which represent approximately one-half to two-thirds of all colorectal polyps and are associated with a higher risk of CRC. Thus, most CRC screening studies evaluate the detection rate of invasive CRC, as well as advanced adenomas, which conventionally are defined as polyps greater than or equal to 10 mm or histologically having high-grade dysplasia or significant villous components. The evidence for the importance of colorectal polyps in the development of CRC is largely indirect, but nonetheless extensive and convincing, and has been described in detail (11–13).
Today there is a range of options for CRC screening in the average-risk population, with current technology falling into 2 general categories: stool tests, which include tests for occult blood or exfoliated DNA; and structural exams, which include flexible sigmoidoscopy (FSIG), colonoscopy, double-contrast barium enema (DCBE), and computed tomographic colonography (CTC). Stool tests are best suited for the detection of cancer, although they also will deliver positive findings for some advanced adenomas, while the structural exams can achieve the dual goals of detecting adenocarcinoma as well as identifying adenomatous polyps (14). These tests may be used alone or in combination to improve sensitivity or, in some instances, to ensure a complete examination of the colon if the initial test cannot be completed. Although screening tests for CRC vary in terms of the degree of supporting evidence, potential efficacy for incidence and mortality reduction, cost-effectiveness, and acceptability, any one of these options applied in a systematic program of regular screening has the potential to significantly reduce deaths from CRC.
Beginning in 1980, the American Cancer Society (ACS) first issued formal guidelines for CRC screening in average-risk adults (15). Since then, the ACS has periodically updated its CRC guidelines (16–19), including adding recommendations for high-risk individuals in 1997 (17). Other organizations also have issued recommendations for CRC screening, most notably the US Preventive Services Task Force (20,21), the American College of Radiology (ACR) (22,23), and the US Multi-Society Task Force on Colorectal Cancer (USMSTF) (12,24). Recently, the ACS and the USMSTF collaborated on an update of earlier recommendations for postpolypectomy and post-CRC resection surveillance in response to reports suggesting significant deviation from existing recommendations (25,26). Since 1997, the organizational guidelines for average-risk adults have grown increasingly similar and represent a broad organizational consensus on the value, options, and methods for periodic screening for CRC.
In the last decade, there has been an increase in the number of technologies available for CRC screening, and in the case of stool tests, there has been growth in the number of commercial versions of guaiac-based and immunochemical-based stool tests (gFOBT and FIT). This growth in options also has been accompanied by changing patterns in the proportion of adults using different tests, with FSIG rates declining, colonoscopy rates increasing, use of stool blood tests remaining somewhat constant, and use of the DCBE for screening now becoming very uncommon (8).
There are pros and cons to having a range of options for CRC screening. Despite the fact that the primary barriers to screening are lack of health insurance, lack of physician recommendation, and lack of awareness of the importance of CRC screening (27), the historical evidence shows that adults have different preferences and patterns of use among the available CRC screening tests (28–31). Although population preferences or resistance to a particular technology may change over time or may be influenced by referring physicians, it also may be true that over time some adults may persist in choosing one technology and rejecting another. Furthermore, at this time not all options are available to the entire population, and transportation, distance, and financial barriers to some screening technologies may endure for some time. Although in principle all adults should have access to the full range of options for CRC screening, the fact that simpler, lower-cost options are available in most settings, whereas other more costly options are not universally available, is a public health advantage. However, for average-risk adults, multiple testing options challenge the referring physician to support an office policy that can manage a broad range of testing choices, their follow-up requirements, and shared decision making related to the options. Shared decision making for multiple screening choices is both demanding and time consuming and is complicated by the different characteristics of the tests and the test-specific requirements for individuals undergoing screening (31). In addition, the description of benefits is complicated by different performance characteristics of the variants of the occult blood tests and uncertain differences between test performance in research settings and test performance in clinical practice. These challenges have been discussed in the past (19,32), and they still are with us today.
In this guideline review, we have reassessed the individual test evidence and comparative evidence for stool tests, including gFOBT, FIT, and stool DNA test (sDNA), and the structural exams, including FSIG, colonoscopy, DCBE, and CTC, the latter also known as virtual colonoscopy. We have sought to address a number of concerns about the complexity of offering multiple screening options and the degree to which the range of screening options and their performance, costs, and demands on individuals poses a significant challenge for shared decisions. An overriding goal of this update is to provide a practical guideline for physicians to assist with informed decision making related to CRC screening. These guidelines are for individuals at average risk. Individuals with a personal or family history of CRC or adenomas, inflammatory bowel disease, or high-risk genetic syndromes should continue to follow the most recent recommendations for individuals at increased or high risk (24–26).
Guidelines, Development, Methods, and Framework
The guidelines update process was divided into 2 phases. The first phase focused on the stool tests, including gFOBT, FIT, and sDNA. The second phase of the guidelines update process focused on the structural exams, including FSIG, colonoscopy, DCBE, and CTC. Deliberations about evidence and presentations from experts took place during 2 face-to-face meetings of the the collaborating organizations and invited outside experts and through periodic conference calls. The process relied on earlier evidence-based reviews (12,16–21,24). Literature related to CRC screening and specific to individual tests published between January 2002 and March 2007 was identified using MEDLINE (National Library of Medicine) and bibliographies of identified articles. Expert panel members also provided several unpublished abstracts and manuscripts. Where evidence was insufficient or lacking to provide a clear, evidence-based conclusion, final recommendations were based on expert opinion and are so indicated.
While there is clear experimental evidence that screening for CRC with gFOBT is associated with reduced incidence and mortality from CRC screening (5,6,33), most of the information supporting the use of the other colorectal screening tests is based on observational and inferential evidence. In this review, priority was placed on studies of asymptomatic average-risk or higher-risk populations that were followed by testing with colonoscopy in all or nearly all study participants as a validation measure.
Summary of the Recommendations
In this update of guidelines for CRC screening in average risk-adults, the expert panel concluded that a screening test must be able to detect the majority of prevalent or incident cancers at the time of testing. Here we are drawing a new, important distinction between test sensitivity and program sensitivity, the former being the sensitivity achieved in a single test and the latter being the sensitivity achieved over time through serial testing in a program. While cancer screening tests are expected to achieve acceptable levels of sensitivity and specificity (34), no specific acceptance threshold for either measure, alone or in combination, has been established for any screening test (35,36). Thus, this criterion is based on expert opinion and the following considerations. First, in the judgment of the panel, recent evidence has revealed an unacceptably wide range of sensitivity among some gFOBT strategies, with some practices and tests performing so poorly that the large majority of prevalent cancers are missed at the time of screening (37–39). The observation of very low sensitivity for cancer and advanced neoplasia associated with in-office gFOBT led Sox to speculate that CRC mortality rates might be considerably lower today if the quality of gFOBT testing during the previous decade had been higher (40). While the literature on other CRC screening tests also reveals a range of sensitivities, even in the presence of significant, correctable, quality-related shortcomings, the majority of invasive cancers still will be detected. Second, a test like gFOBT that demonstrates poor test sensitivity but good program sensitivity depends on high rates of adherence with regular screening. However, many patients have only one test and do not return the following year for programmatic testing (41,42). Given the lack of systems to ensure or at least facilitate adherence with recommended regular screening intervals, as well as evidence of suboptimal awareness and engagement of primary care in supporting adherence with screening recommendations (43), the panel concluded that it was not realistic at this time to rely on program sensitivity to overcome limitations in test sensitivity. Physicians and institutions should select stool blood tests that have been shown in the scientific literature to detect the majority of prevalent CRC in an asymptomatic population. If there is not evidence that an available test has met that benchmark, it should not be offered to patients for CRC screening.
Individuals and health care professionals should also understand that screening tests for CRC broadly fall into 2 categories. In one category are the fecal tests (ie, gFOBT, FIT, and sDNA), which are tests that primarily are effective at identifying CRC. Some premalignant adenomatous polyps may be detected, providing an opportunity for polypectomy and the prevention of CRC, but the opportunity for prevention is both limited and incidental and is not the primary goal of CRC screening with these tests. In the second category are the partial or full structural exams (ie, FSIG, colonoscopy, DCBE, and CTC) (44), which are tests that are effective at detecting cancer and premalignant adenomatous polyps. These tests differ in complexity and accuracy for the detection of CRC and advanced neoplasia. When performed properly, each of these structural exams has met the standard of detecting at least half of prevalent
It is the strong opinion of this expert panel that colon cancer prevention should be the primary goal of CRC screening. Tests that are designed to detect both early cancer and adenomatous polyps should be encouraged if resources are available and patients are willing to undergo an invasive test. These tests include the partial or full structural exams mentioned above. These tests require bowel preparation and an office or hospital visit and have various levels of risk to patients. These tests also have limitations, greater patient requirements for successful completion, and potential harms. Significant positive findings on FSIG, DCBE, and CTC require follow-up colonoscopy.
|Tests that Detect Adenomatous Polyps and Cancer|
|Flexible sigmoidoscopy every 5 years, or
Colonoscopy every 10 years, or
Double-contrast barium enema every 5 years, or
Computed tomographic colonography every 5 years
|Tests that Primarily Detect Cancer|
|Annual guaiac-based fecal occult blood test with high test sensitivity for cancer, or
Annual fecal immunochemical test with high test sensitivity for cancer, or
Stool DNA test with high sensitivity for cancer, interval uncertain
The panel recognized that some patients will not want to undergo an invasive test that requires a bowel preparation, may prefer to have screening in the privacy of their home, or may not have access to the invasive tests due to lack of coverage or local resources. Collection of fecal samples for blood or DNA testing can be performed at home, without bowel preparation. However, providers and patients should understand the following limitations and requirements of noninvasive tests:
- These tests are less likely to prevent cancer compared with the invasive tests;
- These tests must be repeated at regular intervals to be effective;
- If the test is abnormal, an invasive test (colonoscopy) will be needed.
If patients are not willing to have repeated testing or have colonoscopy if the test is abnormal, these programs will not be effective and should not be recommended.
Based on our review of the historic and recent evidence, the tests in Table 1 are acceptable options for the early detection of CRC and adenomatous polyps for asymptomatic adults aged 50 years and older (also see Table 2).
|The following options are acceptable choices for colorectal cancer screening in average-risk adults beginning at age 50 years. Since each of the following tests has inherent characteristics related to prevention potential, accuracy, costs, and potential harms, individuals should have an opportunity to make an informed decision when choosing one of the following options.
In the opinion of the guidelines development committee, colon cancer prevention should be the primary goal of colorectal cancer screening. Tests that are designed to detect both early cancer and adenomatous polyps should be encouraged if resources are available and patients are willing to undergo an invasive test.
|Tests that Detect Adenomatous Polyps and Cancer|
|Test||Interval||Key Issues for Informed Decisions|
|FSIG with insertion to 40 cm or to splenic flexure||Every 5 years||
|Colonoscopy||Every 10 years||
|DCBE||Every 5 years||
|CTC||Every 5 years||
|Tests that Primarily Detect Cancer|
|Test||Interval||Key Issues for Informed Decisions|
|gFOBT with high sensitivity for cancer||Annual||
|FIT with high sensitivity for cancer||Annual|
|sDNA with high sensitivity for cancer||Interval uncertain||
|Abbreviations: FSIG, flexible sigmoidoscopy; DCBE, double-contrast barium enema; CTC, computed tomography colonography; gFOBT, guaiac-based fecal occult blood test; FIT, fecal immunochemical test; sDNA, stool DNA test.|
Screening Tests for the Detection of CRC
Stool blood tests—gFOBT and FIT
Stool blood tests are conventionally known as fecal occult blood tests (FOBT) because they are designed to detect the presence of occult blood in stool. FOBT fall into 2 primary categories based on the detected analyte: gFOBT and FIT. Blood in the stool is a nonspecific finding but may originate from CRC or larger (>1 to 2 cm) polyps. Because small adenomatous polyps do not tend to bleed and bleeding from cancers or large polyps may be intermittent or simply not always detectable in a single sample of stool, the proper use of stool blood tests requires annual testing that consists of collecting specimens (2 or 3, depending on the product) from consecutive bowel movements (18,24,45). FIT generally are processed only in a clinical laboratory, whereas gFOBT are processed either in the physician’s office or in a clinical laboratory. When performed for CRC screening, a positive gFOBT or FIT requires a diagnostic workup with colonoscopy to examine the entire colon in order to rule out the presence of cancer or advanced neoplasia.
gFOBT are the most common stool blood tests in use for CRC screening and the only CRC screening tests for which there is evidence of efficacy from prospective, randomized controlled trials. Guaiac-based tests detect blood in the stool through the pseudoperoxidase activity of heme or hemoglobin, while immunochemical- based tests react to human globin. The usual gFOBT protocol consists of collecting 2 samples from each of 3 consecutive bowel movements at home. Prior to testing with a sensitive guaiac-based test, individuals usually will be instructed to avoid aspirin and other nonsteroidal anti-inflammatory drugs, vitamin C, red meat, poultry, fish, and some raw vegetables because of diet-test interactions that can increase the risk of both false-positive and false-negative (specifically, vitamin C) results (46). Collection of all 3 samples is important because test sensitivity improves with each additional stool sample (14).
gFOBT—Efficacy and Test Performance. Three large, prospective, randomized controlled trials with gFOBT have demonstrated that screened patients have cancers detected at an early and more curable stage than unscreened patients. Over time (8 to 13 years), each of the trials demonstrated significant reductions in CRC mortality of 15% to 33% (5,6,34). Moreover, incidence reduction of 20% was demonstrated in one trial (Minnesota) after 18 years of follow up, which has been attributed to relatively higher rates of colonoscopy in the study (38% of subjects in the screened group) (7).
The sensitivity and specificity of a gFOBT has been shown to be highly variable and varies based on the brand or variant of the test (47); specimen collection technique (38); number of samples collected per test (14); whether or not the stool specimen is rehydrated (ie, adding a drop of water to the slide window before processing) (48); and variations in interpretation, screening interval, and other factors (46).
The reported sensitivity of a single gFOBT varies considerably. In a review by Allison and colleagues, sensitivity for cancer ranged from 37.1% for unrehydrated Hemoccult II to 79.4% for Hemoccult SENSA (47). Lieberman and Weiss compared one-time testing with rehydrated Hemoccult II and observed 35.6% sensitivity for cancer (14). In a study comparing gFOBT (unrehydrated Hemoccult II) with sDNA, sensitivity for cancer was only 12.9% (37). More recently, Allison and colleagues compared a high-sensitivity gFOBT (Hemoccult SENSA) with an FIT and observed 64.3% sensitivity for cancer and 41.3% for advanced adenomas (49). Thus, the data reveal a range of performance among gFOBT variants that allows them to be grouped into low- and high-test sensitivity groups. The specificity of gFOBT also is variable, with low-test sensitivity gFOBT (such as Hemoccult II) tending to have very high specificity and high-test sensitivity gFOBT (such as Hemoccult SENSA) having lower specificity. In a comparison of various stool blood tests, Allison and colleagues observed specificity for cancer and advanced adenomas of 97.7% and 98.1%, respectively, for Hemoccult II, with a combined specificity for cancer and advanced adenomas of 98.1%. For Hemoccult SENSA, which had greater sensitivity for cancer and advanced adenomas compared with Hemoccult II, specificity for cancer and advanced adenomas was 86.7% and 87.5%, respectively, with a combined specificity for cancer and advanced adenomas of 87.5% (47).
A significant limitation of the potential of testing with gFOBT is that it is commonly performed in the doctor’s office as a single-panel test following a digital rectal exam (39). In a recent national survey of primary care physicians, 31.2% reported using only the in-office method of gFOBT, and an additional 41.2% of physicians reporting using both the in-office method or the take-home method. While this approach may seem pragmatic, Collins et al demonstrated that sensitivity is only 4.9% for advanced neoplasia and only 9% for cancer (38). The accuracy of this method is so low that it cannot, under any circumstances or rationale of convenience, be endorsed as a method of CRC screening.
An additional limitation observed in the current use of gFOBT is inadequate follow up of a positive test. Despite the fact that all existing CRC screening guidelines recommend colonoscopy follow up of a positive gFOBT, in the same survey that revealed high rates of in-office gFOBT, nearly one-third of physicians reported that they followed up a positive gFOBT with a repeat gFOBT, and a substantial percentage reported that they referred patients to sigmoidoscopy rather than colonoscopy after a positive gFOBT. Similar patterns of testing and responses to positive test results have been reported by patients undergoing at-home screening (39).
gFOBT—Benefits, Limitations, and Harms. Annual testing with gFOBT has been shown to reduce both CRC mortality and incidence. Testing for occult blood is simple and is associated with minimal harms, although any testing with gFOBT is associated with a possibility of a positive test result that will require follow up with colonoscopy, which is associated with a greater risk of harms. The limitation of gFOBT is that many of the individual tests have limited test sensitivity under the best of circumstances, and this sensitivity may be further compromised by poor and incomplete specimen collection and inadequate or improper processing and interpretation. Program sensitivity (ie, the outcome of repeat annual testing) is considerably higher, but the systems to ensure regular, annual testing often are not in place to support either the patient or his or her physician to be adherent. Further, testing in the office following a digital rectal exam, which is highly inaccurate, has been common and still may persist at significant levels today. When either the test, the testing procedure, or both have very low test sensitivity and when positive tests are not followed up with colonoscopy, the potential is high for patients to have a false sense of reassurance after testing. Finally, patients who choose gFOBT for CRC screening must understand that annual testing is required.
Quality Assurance. If patients and their providers select gFOBT for CRC screening, they should be aware of several quality issues based on programmatic performance in clinical trials. First, the test must be performed properly with 3 stool samples obtained at home. A single-stool sample FOBT collected after digital rectal exam in the office is not an acceptable screening test, and it is not recommended. Prior to testing with a sensitive guaiac-based test, individuals should be instructed to avoid nonsteroidal anti-inflammatory drugs such as ibuprofen, naproxen, or aspirin (more than one adult aspirin per day) for 7 days prior to testing unless they are on a cardioprotective regimen. There has been debate as to whether additional dietary restrictions reduce compliance with testing and are necessary to reduce the risk of both false-negative and false-positive results. Results of a meta-analysis that examined completion and positivity results found little support for the influence of dietary restrictions on completion or positivity rates, with the exception of completion rates in one study that imposed severe restrictions. However, manufacturers still endorse avoidance of vitamin C in excess of 250 mg from either supplements or citrus fruits and juices and avoidance of red meats (beef, lamb, and liver) for 3 days before testing. This seems prudent since recent consumption of red meat is associated with increased false positivity, and excess vitamin C can result in false-negative results. Second, it is critically important that physician offices and laboratories follow recommended quality-assurance procedures for test development and interpretation. Although rehydration of gFOBT slides increases sensitivity, it is not recommended because it can adversely affect the readability of the test and also substantially increases the false-positive rate. Sinatra and colleagues observed considerable variation in the interpretation of gFOBT among 13 laboratories in Melbourne, Australia, and concluded that ongoing technician training and review of laboratory procedures were important (50). Better results may be achieved if guaiac-based tests are routinely processed and interpreted in a clinical laboratory. Third, if the test is positive, patients should be advised to have colonoscopy. Repeating the stool test or follow up with noncolonoscopy tests are inappropriate. Fourth, if the test is negative, patients should understand that they need to have repeated testing annually.
gFOBT—Conclusions and Recommendations. Annual screening with high-sensitivity gFOBT (such as Hemoccult SENSA) that have been shown in the published, peer-reviewed literature to detect a majority of prevalent CRC in an asymptomatic population is an acceptable option for colorectal screening in average-risk adults aged 50 years and older. Any positive test should be followed up with colonoscopy. Individuals should be informed that annual testing is necessary to achieve the fullest potential of this test and that they will need follow-up colonoscopy if test results are positive. Screening for CRC with gFOBT in the office following digital rectal exam or as part of a pelvic examination is not recommended and should not be done. Commonly used guaiac tests, with or without rehydration, that have not been shown in the literature to detect a majority of prevalent CRC at the time of testing are no longer recommended.
The concept of applying an immunochemical method to testing stool for occult blood was first proposed in the 1970s (51), and commercialization of the technology began in the 1980s. The use of FIT in the United States has lagged behind some other countries, mostly due to the higher costs associated with FIT compared with gFOBT. However, recently increased reimbursement by Medicare made the use of FIT financially viable and has led to its wider acceptability in the United States (52).
FIT has several technological advantages when compared with gFOBT. FIT detects human globin, a protein that along with heme constitutes human hemoglobin. Thus, FIT is more specific for human blood than guaiac-based tests, which rely on detection of peroxidase in human blood and also react to the peroxidase that is present in dietary constituents such as rare red meat, cruciferous vegetables, and some fruits (53). Further, unlike gFOBT, FIT is not subject to false-negative results in the presence of high-dose vitamin C supplements, which block the peroxidase reaction. In addition, because globin is degraded by digestive enzymes in the upper gastrointestinal tract, FIT also are more specific for lower gastrointestinal bleeding, thus improving their specificity for CRC. Finally, the sample collection for some variants of FIT are less demanding of patients than gFOBT, requiring fewer samples or less direct handling of stool.
FIT—Efficacy and Test Performance. Recently, a number of new FIT have entered the market, although not all are available in the United States. Some of the new FIT have been evaluated in comparison with gFOBT in diagnostic accuracy studies with human subjects who all undergo colonoscopy to define the true presence or absence of neoplasia. Other FIT have been evaluated only on the basis of their ability to detect the presence of certain concentrations of blood in laboratory settings. No FIT has been tested in a randomized trial where the outcome of interest is CRC mortality, nor is it likely, as is the case with colonoscopy, that such a study will ever be undertaken.
A number of studies over the past 20 years have compared the diagnostic accuracy of various FIT with gFOBT (most often Hemoccult II or Hemoccult SENSA). In this review, we have focused on studies that compared different FIT with Hemoccult SENSA since at present it has the highest sensitivity of currently marketed gFOBT (49,54–58). Based on data from these 6 studies, it appears that there are no clear patterns of superior performance in overall test performance between a high-sensitivity guaiac-based test (Hemoccult SENSA) and a variety of FIT.
FIT has been performed in subjects undergoing screening colonoscopy to determine one-time sensitivity and specificity. Morikawa et al studied 21,805 asymptomatic adults who underwent testing with the Magstream 1000 test (not available in the United States), followed by colonoscopy (59). The Magstream FIT was positive in 5.6% of patients, with 27.1% sensitivity for advanced neoplasia and 65.8% sensitivity for cancer. In a similar study, although not in a totally asymptomatic population, Levi and colleagues sought to measure both sensitivity and specificity of a quantitative FIT and, as well, to measure fecal hemoglobin thresholds most predictive of advanced neoplasia and cancer (58). One thousand ambulatory patients, some with and some without symptoms of CRC, who were scheduled for colonoscopy and who were willing to also undergo an FIT with 3 samples were included in the study. The hemoglobin content of 3 bowel movements was measured. The sensitivity for cancer with 3 FIT samples with a hemoglobin threshold set at 75 ng/mL was 94.1%. Specificity for cancer was 87.5%. Allison and colleagues recently published results of a comparison of a sensitive gFOBT (Hemoccult SENSA) with a FIT (Hemoccult ICT) for cancer and advanced adenomas in the distal colon in nearly 6,000 average-risk subjects who had undergone FSIG (49). Both tests showed superior sensitivity for cancer compared with the single-test performance of an unrehydrated gFOBT. The sensitivity for CRC of the FIT and the sensitive gFOBT was 81.8% and 64.3%, respectively. However, the sensitive gFOBT showed superior performance for advanced adenomas (41.3%) compared with FIT (29.5%). Specificity of FIT tends to be higher than that observed for high-sensitivity gFOBT. For example, in the analysis by Allison et al, the specificity of Hemoccult ICT was 96.9% for distal cancer, 97.3% for distal advanced lesions, and 97.5% for all distal advanced neoplasia (49).
FIT—Benefits, Limitations, and Harms. The spectrum of benefits, limitations, and harms is similar to a gFOBT with high sensitivity. One advantage of FIT over gFOBT appears to be a function of fewer demands on patients undergoing FIT compared with gFOBT. FIT does not require a restricted diet, and the sampling procedures for some forms of FIT are less demanding (60). Quality Assurance. If patients and their providers select FIT, they should be aware of several quality issues. Although there are no clinical trials assessing programmatic performance, an effective screening program will depend on repeat testing if the initial test is negative and referral for colonoscopy if the test is positive. At this time, the optimal number of FIT stool samples is not established, but 2 samples may be superior to one (61).
FIT—Other Issues. Given the lack of clear difference in test performance in studies conducted to date, policy makers, providers, and patients may want to consider other factors when deciding which occult blood test to use. Relevant other factors include cost (both out-of-pocket and total costs) and likelihood of test completion, which appears to be greater with FIT compared with gFOBT (60).
FIT—Conclusions and Recommendations. Annual screening with FIT that have been shown in the published, peer-reviewed literature to detect a majority of prevalent CRC in an asymptomatic population at the time of testing is an acceptable option for colorectal screening in average-risk adults aged 50 years and older. Any positive test should be followed up with colonoscopy. Adults should be informed that annual testing is necessary to achieve the fullest potential of this test and that they will need follow-up colonoscopy if test results are positive.
Knowledge of molecular genomics provides the basis of a new method of CRC screening that tests stool for the presence of known DNA alterations in the adenoma-carcinoma sequence of colorectal carcinogenesis. Adenoma and carcinoma cells that contain altered DNA are continuously shed into the large bowel lumen and passed in the feces. Because DNA is stable in stool, it can be differentiated and isolated from bacterial DNA found in the feces (62). No single gene mutation is present in cells shed by every adenoma or cancer. Thus, a multitarget DNA stool assay is required to achieve adequate sensitivity. At present there is only one commercially available sDNA test. The prototype assay of this test (version 1.0) for which most of the published evidence is available consisted of a multiple-marker panel that included 21 separate point mutations in the K-ras, APC, P53 genes; a probe for BAT-26 (a marker of microsatellite instability); and a marker of DNA integrity analysis (DIA). The sDNA that is currently commercially available is a second-generation version of this test (version 1.1) that includes this same marker panel but incorporates several technical advances related to processing and specimen preservation. (63,64) Whereas gFOBT and FIT test a sample of stool or sample of water surrounding stool, the currently available sDNA test requires the entire stool specimen (30 g minimum to ensure an adequate sample of stool for evaluation). Collection kits have been designed to facilitate specimen collection and mailing and to enhance compliance.
sDNA—Efficacy and Test Performance. Several studies on the sensitivity and specificity of sDNA testing for CRC detection have been published utilizing a panel of DNA markers (37,65–69). Test sensitivity for CRC in these studies ranged from 52% to 91%, with specificity ranging from 93% to 97%. Lower sensitivity in some of these studies has been attributed to suboptimal sensitivity performance of DIA resulting from DNA degradation during transit of specimens to the laboratory. The changes associated with version 1.1 are reported to address these problems. One study utilizing version 1.1 has been published by Whitney et al (63) reporting a sensitivity for CRC of 70%.
sDNA has been compared to a low-sensitivity gFOBT in one large, prospective study of an average-risk screening cohort. Imperiale et al conducted an investigation in a cohort of 2,507 average-risk individuals undergoing colorectal neoplasia screening by 3 modalities: sDNA using the prototype assay (version 1.0), gFOBT (nonrehydrated Hemoccult II), and colonoscopy (37). sDNA testing had statistically significantly better sensitivity for CRC compared with Hemoccult II (52% versus 13%) and for all cancers and high-grade dysplasia (40.8% versus 14.1%), with comparable specificity. In this study, sDNA was much less sensitive in the detection of all advanced adenomas (15.1%), defined as a tubular adenoma at least 1 cm in diameter, an adenoma with a villous histologic appearance, or an adenoma with high-grade dysplasia, although it still showed superior performance to the comparison gFOBT (10.7%) (37). Data on program performance of sDNA screening are lacking. Information on the sensitivity and specificity of CRC and adenoma detection comes from an evaluation of results from a single test. Also, the currently available sDNA gene test—version 1.1—has not been rigorously tested in screening cohorts but based on available data can be reasonably assumed to perform as well or better than version 1.0 (63). New version assays with better DNA stabilization and simplified genetic analyses may be more sensitive than version 1.0 but require testing in screening cohorts (70).
sDNA—Benefits, Limitations, and Harms. The primary benefit of sDNA is that this methodology has acceptable sensitivity for CRC and is built upon the concept of detecting molecular markers associated with advanced colorectal neoplasia. It is not dependent on the detection of occult bleeding, which is intermittent and nonspecific, and it requires only a single stool collection. Further, newer versions may have better sensitivity as more is learned about markers that are common across all prevalent CRC, as well as advanced adenomas. sDNA sampling also is noninvasive and lacks physical harm. Patient and provider acceptance of this technique appears to be high, with available data indicating that sDNA is preferred over other tests by some individuals, and among others testing with sDNA, it is at least as acceptable to patients as testing with gFOBT (29,71). Berger et al reported that most individuals undergoing sDNA who completed a mailed survey reported satisfaction with the sDNA testing process, and most reported that they would repeat testing if recommended by their physician (72).
A clear limitation of sDNA testing for the detection of CRC and large adenomas is that test sensitivity is based on a panel of markers that appears to identify the majority of but not all CRC. Further, it is not known what proportion of advanced adenomas is identified with the current commercial version (version 1.1) of the sDNA test. Other potential limitations that have considerable implications for cost-effectiveness are the unit cost of the current test (73), which is much higher than the other stool tests, and the frequency with which the test should be performed, which is uncertain. Currently, the test is under review by the Food and Drug Administration for 510K certification but is commercially available under the "home brew" category.
An additional issue is the clinical relevance of a positive genetic test without identification of the cause of the abnormality; this has not been studied systematically. At issue for a test that is based on molecular markers is the degree to which a positive test, with no evidence of advanced lesions upon completion of colonoscopy, is truly negative or positive for a lesion that is not yet clinically evident. Osborn and Ahlquist have highlighted the fact that inasmuch as cancers exfoliate cells and that these cells can survive the digestive process and ultimately be excreted in stool, high prevalence supracolonic aerodigestive cancers may also be detected by sDNA (74). However, at this time, the significance of a positive test result in a patient with a negative follow-up evaluation is unknown.
Quality Assurance. Individuals should be informed about the benefits and limitations of screening for CRC with sDNA, including the facts that at present the test is more sensitive for cancer than advanced adenomas, that the current panel of markers will not identify all cancers, and that a positive test will need to be followed up with colonoscopy. Individuals should also know that the rescreening interval after a negative test is uncertain. Individuals should be made aware that their stool specimen must be packaged and shipped in a customized collection kit that includes a specially designed ice pack. Patients must have access to a working freezer and allow this ice pack to freeze for at least 8 hours prior to use. If the specimen is returned without the ice pack or if there are unforeseen delays in specimen return or processing, the specimen may be rejected.
sDNA—Other Issues. Testing stool for mutated DNA and other markers poses unique challenges in shared decision making. The panel of markers that was evaluated in population studies was not sensitive for all advanced lesions and cancer, and there is uncertainty about improvements in the sensitivity of newer versions for advanced neoplasia and cancer in screening cohorts. At this time, patients will need to be informed that sDNA will detect some but not all advanced lesions and cancers. There also is uncertainty about how positive results without evidence of advanced lesions or cancer on follow up should be interpreted by patients and whether or not these patients require a different plan for ongoing surveillance (75). Additional research is necessary to resolve these questions.
As noted previously, the most informative data on the performance of sDNA is from version 1.0, which has been replaced with version 1.1; the newer version uses the same panel of markers but is reported to have improved quality (63,70). Newer versions are currently under evaluation and are reported to have improved sensitivity, with diminution of specificity. The evolution of tests of this type raises important questions as to how performance of successive iterations should be evaluated and whether large prospective studies of asymptomatic patients with follow-up colonoscopy among all participants are required. Another question worthy of consideration is whether or not including a sensitive gFOBT or FIT at the time of testing would improve sensitivity, without adversely affecting specificity. In a recent retrospective analysis of stool samples from patients with CRC and donor controls, combined results from a standard gFOBT and a panel of DNA markers (APC, BAT-26, and L-DNA) resulted in a combined sensitivity for cancer of 93% and specificity of 89% (76).
sDNA—Conclusions and Recommendations. In previous assessments of the performance of sDNA, both the ACS and the USMSTF concluded that data were insufficient to recommend screening with sDNA for average-risk individuals (19,24). Based on the accumulation of evidence since the last update of these guidelines, the panel concluded that there now are sufficient data to include sDNA as an acceptable option for CRC screening. As noted above, testing stool for molecular markers is an evolving technology. New iterations of these tests, either technological enhancements of existing tests or completely new test variants, should be carefully evaluated in order to determine that they meet the criteria of detecting a majority of cancers at the time of screening but also have acceptable performance in a screening cohort. While the manufacturer of the one test that is commercially available currently is recommending a 5-year interval for routine screening between examinations with normal results, the panel concluded that there were insufficient data upon which to endorse this interval. Such an interval was judged by the committee to be appropriate only for a test that has very high sensitivity for both cancer and adenomatous polyps—a standard that has not been documented for sDNA to date. At this time, further research is needed to determine the interval between negative sDNA exams. Based on current evidence, the appropriate interval is uncertain.
Tests for the Detection of Adenomas and CRC
Endoscopy examinations of the colon and rectum—FSIG and colonoscopy
FSIG is an endoscopic procedure that examines the lower half of the colon lumen. In addition to the standard 60 cm sigmoidoscope, the exam may be performed with a variety of endoscopic instruments, including a colonoscope, an upper endoscope, and a pediatric colonoscope. It is typically performed without sedation and with a more limited bowel preparation than standard colonoscopy. Since sedation is not required, it can be performed in office-based settings and by nonphysicians, including nurses or physician assistants, provided adequate training has been received (77).
FSIG—Efficacy and Test Performance. The use of FSIG for CRC screening is supported by high-quality case-control and cohort studies, which have been reviewed in detail elsewhere (24). In 2 well-known case-control studies, FSIG was associated with a 60% to 80% reduction in CRC mortality for the area of the colon within its reach, and this protective effect appears to persist for 10 years or more (4,78). A small, randomized trial (79) and a case-control study (80) also demonstrated decreased CRC incidence in the sigmoidoscopy-screened group compared with a nonscreened control group. There are 4 prospective, randomized controlled trials ongoing in the United States and Europe (81–84), and results are expected in the near future.
Additional evidence supporting the effectiveness of FSIG derives from colonoscopy studies. FSIG is 60% to 70% as sensitive for advanced adenomas and cancers in the colon compared with colonoscopy (85,86). However, this figure varies according to age, with proximal neoplasia becoming more common after age 65 years (87). Due to observed differences in the distribution of colonic neoplasia, FSIG may also be less sensitive in women than in men (88), although the overall prevalence of advanced colonic neoplasia is lower in women than in men (89), and it may be less sensitive in African Americans than in Whites. Several studies have indicated that African Americans have a higher prevalence of proximal lesions than Whites (90,91), although a more recent evaluation of proximal lesions in a consecutive series of African American and White adults undergoing FSIG did not observe a statistically significant difference in proximal lesions between the 2 groups based among those adults with neoplastic lesions identified during sigmoidoscopy (92). In addition, a number of recent studies have documented a lower prevalence of distal colon and rectal lesions in Whites compared with Hispanics and Asians (92,93). Differences in the prevalence of distal and proximal lesions based on age, gender and ethnicity, and the benefits and limitations of CRC screening with FSIG among these different groups remain important areas for continued investigation.
The effectiveness of sigmoidoscopy depends on the completion of a high-quality exam. Studies have demonstrated variable adenoma detection rates at screening sigmoidoscopy that are attributed to exam quality and completeness (94). Advanced neoplasia has been found within 3 years of a negative sigmoidoscopy in the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial, raising issues of exam quality (95). Although scope insertion to beyond 40 cm is only one measure of quality, the clinical studies that report adenoma detection and efficacy all achieve this level of insertion (96). Studies have demonstrated that deeper levels of insertion are associated with a higher detection rate for advanced neoplasia (86). Therefore, the panel recommends that if sigmoidoscopy is performed for CRC screening,
Exam quality also depends on the appropriate management of endoscopic findings. The panel recommends that any endoscopist performing sigmoidoscopy should be skilled in obtaining biopsies of polyps to determine histology. The histologic findings are informative for follow-up decision making. There is evidence from 2 large screening studies that if a patient has an adenoma of any size in the distal colon, he or she has an increased risk of proximal advanced neoplasia (2-fold or higher) compared with patients who have no polyps or only hyperplastic polyps in the distal colon (14,85). Therefore, we recommend that most patients who have adenomas discovered at sigmoidoscopy should undergo colonoscopy. If biopsies are not obtained, another strategy is to refer all patients with one >5 mm for colonoscopy (97).
The appropriate interval between normal sigmoidoscopy exams is uncertain and may extend to 10 years, although the protective effect would depend greatly on the quality of the examination. Prior ACS and USMSTF CRC screening guidelines have recommended a 5-year interval between normal FSIG examinations, while recommending a 10-year interval between colonoscopy examinations (18,24). The shorter interval was recommended for FSIG because of concerns about exam quality and completeness in most clinical settings. In settings where an experienced endoscopist performs a complete examination on a well-prepared patient and achieves insertion beyond 40 cm, a 10-year interval between screening FSIG may be justified. Since, these criteria are not routinely achieved in many clinical settings, a 5-year rescreening interval remains the standard recommendation.
The most important limitation in the evidence for FSIG is the lack of a longitudinal head-to-head comparison between FSIG screening and other CRC screening tests, such as colonoscopy or the different stool blood tests. Apart from the issue of patient preference, a key question for screening policy is the incremental benefit of colonoscopy over FSIG, given the higher direct medical and indirect costs of colonoscopy and the higher risk of complications with colonoscopy (98).
FSIG—Benefits, Limitations, and Harms. The chief advantage of FSIG is that it can be performed with a simple preparation (2 Fleet enemas), without sedation, and by a variety of examiners in diverse settings. With respect to distal bowel cleansing, the use of enemas is often imperfect, and superior bowel cleansing is achieved with the more thorough oral sodium phosphate procedure. Patients have reported a more favorable experience with the oral prep compared with the enemas (99). The absence of sedation is perceived by some patients as an advantage and by others as a disadvantage, although in one series, a greater percentage of patients undergoing sigmoidoscopy reported periprocedural discomfort (during and postexam) compared with patients undergoing colonoscopy (100). Moreover, lack of sedation is associated with greater patient discomfort and greater patient reluctance to undergo the examination for future screening (100).
An additional limitation of FSIG is that there may be considerable variation both in depth of insertion of the scope and in adenoma detection at FSIG between different examiners (94,101), and this may reduce the effectiveness of FSIG for CRC screening, especially in practice settings of low volume. Quality assurance is an important issue for flexible sigmoidoscopists and has been reviewed in detail elsewhere (77). Providers should be well trained and should exceed the published American Society for Gastrointestinal Endoscopy standards for a minimum number of training examinations prior to performing sigmoidoscopy
The chief limitation of FSIG is that it does not examine the entire colon but, under optimal conditions, only the rectum, sigmoid, and descending colon. However, several lines of evidence support the idea that the incremental benefit of colonoscopy is less than simply the difference in sensitivity for advanced adenomas between colonoscopy and FSIG because many patients with small distal adenomas will receive colonoscopy, which may result in discovery of proximal advanced adenomas and cancer. The complications of FSIG include colonic perforation, even if no biopsy or polypectomy is performed, but this occurs in fewer than one in 20,000 examinations.
Quality Assurance. Quality indicators for FSIG have been previously published.77 Key elements include 1) appropriate training of endoscopists; 2) satisfactory examination rates to beyond 40 cm; 3) expected adenoma detection rates based on age and gender; and 4) ability to biopsy suspected adenomas. The effectiveness of an FSIG program is based on the assumption that if an adenoma is detected in the sigmoid colon or rectum, the patient would be referred for total colonoscopy. Patients should fully understand that in most circumstances colonoscopy will be recommended if an adenoma is detected during FSIG and that if they are unwilling to accept referral to colonoscopy, they should have a different form of screening.
FSIG—Other Issues. FSIG use in the United States has been decreasing in the recent decade, coincident with a rise in colonoscopy usage. An analysis of Medicare data from the years 1993 to 2002 demonstrated a 54% decrease in sigmoidoscopy use between the earliest and latest periods studied and a more than 6-fold increase in colonoscopy usage over the same time frame (103). Other data from endoscopic facilities across the United States collected and analyzed by investigators from the Centers for Disease Control and Prevention estimated that approximately 2.8 million FSIG examinations and 14.2 million colonoscopy examinations were performed in 2002 (104). Low reimbursement and a shortage of adequately trained examiners are 2 barriers to the availability of FSIG (30,105). In settings where reimbursement rates have not been a concern and where nurse endoscopists have been employed, high rates of FSIG utilization have been achieved (106).
FSIG—Conclusion and Recommendations. FSIG can result in the identification of the majority of prevalent CRC at the time of screening, when the examination reaches the splenic flexure or beyond 40 cm as a reasonable target for insertion and when adenomas in the distal colon are used as an indication for the need for colonoscopy. Although the appropriate interval between normal examinations is uncertain, FSIG is recommended to be performed for screening every 5 years in most clinical settings due to concerns about exam quality and completeness. FSIG can be performed alone, or consideration can be given to combining FSIG performed every 5 years with a highly sensitive gFOBT or FIT performed annually. In high-quality centers (such as the program operated by Kaiser Permanente in California) where procedures are conducted by properly trained and experienced endoscopists who document regular insertion beyond 40 cm with a good bowel preparation, a 10-year interval between negative exams may be reasonable.
Individuals should be informed about the limitations of FSIG, including the fact that it examines only the distal colon; that there is a risk, albeit small, of perforation; and that they may experience discomfort during and after the examination. Patients should also understand that the examination achieves higher quality when bowel cleansing follows the same protocol as that for colonoscopy. Finally, patients should be informed that positive test findings will need to be followed up with colonoscopy.
Colonoscopy is one of the most commonly performed medical procedures in the United States, with estimates of up to 14 million procedures performed in 2003 (104). Colonoscopy allows direct mucosal inspection of the entire colon from the appendiceal orifice to the dentate line and same-session biopsy sampling or definitive treatment by polypectomy in the case of precancerous polyps and some early-stage cancers. The modern colonoscope is capable of examining the entire bowel, with the examination terminating at the cecum. Patients generally adopt a liquid diet one or more days before the examination, followed by either ingestion of oral lavage solutions or saline laxatives to stimulate bowel movements until the bowel is clean. Proper bowel preparation is a critical element in the accuracy and cost-effectiveness of screening with colonoscopy (107). It is common for the patient to receive a mild sedative prior to the procedure, but it is not essential for those who tolerate the procedure with only mild discomfort (108).
Colonoscopy—Efficacy and Test Performance. There are no prospective randomized controlled trials of screening colonoscopy for the reduction in incidence or mortality of CRC; however, because colonoscopy is used to evaluate other positive screening tests, there is evidence to indicate that colonoscopy and polypectomy result in incidence reductions in randomized controlled trials of other screening tests. The University of Minnesota randomized controlled trial of FOBT observed a 20% reduction in incidence of CRC, which the authors attribute to colonoscopy and polypectomy in patients with a positive FOBT (7). In a randomized controlled trial of FSIG versus no screening and with follow- up colonoscopy and polypectomy performed for any polyp detected at sigmoidoscopy, the screening group experienced an 80% incidence reduction in CRC (79).
Case-control studies cited above of sigmoidoscopy and polypectomy in screening populations also are considered to provide supporting evidence for colonoscopy because of the similarity of the examinations in the distal colon. In a case-control study of colonoscopy in the US VA population, colonoscopy in symptomatic patients was associated with a 50% reduction in mortality (109).
The evaluation of incidence rates of CRC in adenoma cohorts after baseline colonoscopy and polypectomy is another form of evidence commonly cited to support colonoscopy for CRC screening. In the National Polyp Study, the incidence of CRC after clearing colonoscopy was reduced by 76% to 90% compared with 3 nonconcurrent reference populations (110). In an Italian adenoma cohort study with removal of at least one adenoma ≥5 mm, there was an 80% reduction in CRC incidence compared with expected incidence in a reference population (111). However, not all studies have shown the same level of protection. Combined data from 3 US chemoprevention trials showed incidence rates of CRC after clearing colonoscopy approximately 4 times that seen in the National Polyp Study, with no reduction in CRC incidence compared with data from the Surveillance Epidemiology and End Results (SEER) database in the United States, 112 and 2 US dietary intervention trials also showed higher rates of incident CRC after clearing colonoscopy than were observed in the National Polyp Study (113,114). These differences may reflect exclusion of patients with sessile adenomas >3 cm in size in the National Polyp Study, more effective baseline clearing (13% of patients in the National Polyp Study had 2 or more baseline colonoscopy to complete clearing), or unmeasured differences in the average quality of colonoscopy between the studies.
Overall, the data support the conclusion that colonoscopy with clearing of neoplasms by polypectomy has a significant impact on CRC incidence and, thus, by extension, mortality. The magnitude of the protective impact is uncertain; it is not absolute, nor are apparent failures well understood. In a study of 35,000 symptomatic patients in Manitoba who had undergone a negative colonoscopy and who then were followed for 10 years, the investigators observed significant reductions in CRC incidence over time, but the incidence reductions were less than 50% for each of the first 5 years and no more than 72% by 10 years. These findings suggest detection failures during the initial, apparently normal, colonoscopy.
Colonoscopy—Benefits, Limitations, and Harms. A principal benefit of colonoscopy is that it allows for a full structural examination of the colon and rectum in a single session and for the detection of colorectal polyps and cancers accompanied by biopsy or polypectomy. All other forms of screening, if positive, require colonoscopy as a second procedure.
Patient surveys indicate that patients willing to undergo invasive testing tend to choose colonoscopy as their preferred test (71). In addition to being a complete examination of the colon, individuals may also regard sedation during the procedure as an advantage. Patients in the same practice who had undergone unsedated FSIG screening were more than twice as likely to say that they would not return for additional screening compared with those who had undergone colonoscopy with sedation (100).
Colonoscopy has several limitations. It requires one or more days of dietary preparation and bowel cleansing, usually a day dedicated to the examination, and because of sedation, a chaperone is needed for transportation. It is an invasive procedure, and surveys indicate that a significant percentage of adults prefer other noninvasive options for CRC screening (71,115,116). Effective performance of the procedure is dependent on thorough bowel preparation, which is often perceived as the most unpleasant part of the colonoscopy process by those who have undergone the test. Limitations with regard to detection of neoplasia have been previously discussed, and the fact that colonoscopy is operator-skill dependent is another significant limitation. Patients are generally poorly informed about the problem of variable performance of the procedure and are unaware of the skill level of their endoscopists. Formal quality-assurance programs do not exist, and the current reimbursement system for colonoscopy does not reward careful examination but tends to reward rapidly performed examinations and repeated examinations at unnecessarily short intervals (117). Polypectomy is sometimes ineffective in eradicating polyps, a factor that has been implicated as the cause of up to 25% of interval cancers (118,119). Finally, colonoscopy is not an infallible "gold standard." Controlled studies have shown the colonoscopy miss rate for large adenomas (=10 mm) to be 6% to 12% (120,121). The reported colonoscopy miss rate for cancer is about 5% (120,122).
Colonoscopy can result in significant harms, most often associated with polypectomy, and the most common serious complication is postpolypectomy bleeding. The risk of postpolypectomy bleeding is increased with large polyp size and proximal colon location; however, small polyp bleeds are more numerous than large polyp bleeds because small polyps are so numerous. Another significant risk associated with colonoscopy is perforation. Perforation increases with increasing age and the presence of diverticular disease and was recently estimated to occur in 1 in 500 of a Medicare population and approximately 1 in 1,000 screened patients overall (123). Because of the age effect, perforation rates measured in the Medicare population may overestimate the overall risk of perforation in colonoscopy; however, a large study in the Northern California Kaiser Permanente population also identified a perforation rate of 1 in 1,000.98 In addition, cardiopulmonary complications such as cardiac arrhythmias, hypotension, and oxygen desaturation may occur, although these events rarely result in hospitalization. Cardiopulmonary complications represent about one-half of all adverse events that occur during colonoscopy and usually are related to sedation (124). Thus, while screening colonoscopy has established benefits with regard to the detection of adenomas and cancer, complications related to colonoscopy are a significant public
Quality Assurance. Recent publications have highlighted criteria for best practices and important quality indicators for colonoscopy (124–126). High-quality colonoscopy depends on 1) appropriate training and experience; 2) proper documentation of risk assessment; 3) complete exam to the cecum with adequate mucosal visualization and bowel preparation; 4) ability to detect and remove polyps safely; 5) documentation of polypoid lesions and methods of removal; 6) timely and appropriate management of adverse events; 7) appropriate follow up of histopathology findings; and 8) appropriate recommendation for surveillance or repeat screening based on published guidelines. Although colonoscopy is commonly used for screening, diagnosis, and therapy, until recently there was no standardized reporting system for this procedure. To enhance clear communication about colonoscopy findings between health care professionals and to facilitate quality-improvement programs, the Quality Assurance Task Group of the National Colorectal Cancer Roundtable developed a reporting and data system for colonoscopy based on previously published continuous quality-improvement indicators (126).
Colonoscopy—Other Issues. Colonoscopy in the United States is performed by the overwhelming majority of gastroenterologists, most colorectal surgeons, many general surgeons, and a small percentage of primary care physicians. Colonoscopy volumes have risen steadily in the United States, while volumes for FSIG and DCBE have declined substantially in the past decade, and FOBT has remained relatively stable, although a small decline in the rate was observed among women (8,127). Colonoscopy is offered in the vast majority of American hospitals and is also widely performed in ambulatory surgery centers and in physicians’ offices in some parts of the country. A recent survey of American colonoscopists suggested that capacity could be increased from the present 14 million annual procedures to 22 million with currently available resources (104), although the methodology behind this estimate has been criticized (128), and other estimates of capacity are less optimistic regarding capacity (129,130). In the short term, colonoscopy capacity appears sufficient to handle slow increases in demand for the majority of the US population, although the capacity to handle a sharp increase in demand for screening or diagnostic/therapeutic colonoscopy overall is uncertain and likely highly variable geographically.
Some of the limitations in the availability of colonoscopy for screening potentially could be overcome by more appropriate use of surveillance colonoscopy after polyp resection, which has been shown to be excessive among gastroenterologists and particularly among general surgeons and primary care physicians (117,131). Excessive rates of short-term follow up after polypectomy, especially for small lesions, also likely diminish the cost-effectiveness of colonoscopy. For these reasons, the ACS and USMSTF recently updated and further clarified recommendations for postpolypectomy surveillance (25). The case against serial short-term follow-up strategies rests on observations that over the short term, the risk of significant growth of adenomas is quite low. However, because there is uncertainty about the natural history of small colorectal adenomas and perhaps because of a desire to err on the side of prudence, a significant percentage of clinicians recommends follow-up intervals that are considerably shorter than recommended, and surveillance intervals often are not adjusted for subsequent negative findings (117). Recent guideline recommendations continue to expand the interval between follow-up colonoscopy examinations
Colonoscopy—Conclusions and Recommendations. The evidence base to support screening colonoscopy, though indirect, is substantial. The appropriate interval between negative colonoscopy screening exams is uncertain because of lack of long-term follow-up data. At present, colonoscopy every 10 years is an acceptable option for CRC screening in average-risk adults beginning at age 50 years. Individuals should be informed about the limitations of colonoscopy, including the fact that it may miss some cancers and significant adenomas and that there is a risk, albeit small, of perforation, hemorrhage (following polypectomy), subsequent hospitalization, and in very rare circumstances, more serious harms. A full bowel cleansing is necessary prior to colonoscopy. Sedation usually is used to minimize discomfort during the examination, and thus a chaperone is required to provide transportation after the examination.
Imaging examinations of the colon and rectum—DCBE and computed tomography
The DCBE, sometimes referred to as air-contrast barium enema, evaluates the colon in its entirety by coating the mucosal surface with high-density barium and distending the colon with air introduced through a flexible catheter that is inserted into the rectum. Multiple radiographs are acquired while varying the patient position during direct fluoroscopic evaluation and, subsequently, with conventional radiographic equipment. Colonic preparation, usually a 24-hour dietary and laxative regimen, is essential for an optimal examination. Sedation is not utilized, and the duration of the procedure averages about 20 to 40 minutes. Patients may experience mild to moderate discomfort during and after the procedure, but a prompt return to normal activity is typical.
DCBE was contemporaneously adopted as a CRC screening option by the Multi-Society Gastroenterology Consortium and the ACS in 1997 and has continued to be included among the recommended screening options in periodic updates of those guidelines (12,17,24,132), as well as those of the US Preventive Services Task Force (21). It is also considered appropriate for screening by the ACR.133 CRC screening of the average-risk population with DCBE also has been a designated Medicare benefit since 1997 (134).
DCBE—Efficacy and Test Performance. There have been no randomized controlled trials evaluating the efficacy of DCBE as a primary screening modality to reduce incidence or mortality from CRC in average-risk adults, and there also are no case-control studies evaluating the performance of DCBE. Further, the existing literature describing the test performance of DCBE also is limited by study designs that are retrospective and commonly do not report findings from an asymptomatic or average-risk population (135,136). In some reports, asymptomatic individuals were selected for investigation during neoplasm surveillance or after a prior screening test (eg, FSIG or FOBT). Finally, similar to the literature related to other CRC screening technologies, the DCBE literature varies considerably in terms of measurement and outcome metrics (ie, polyps, cancers, all neoplasms, adenomas, size categorizations, etc.), and these measurements
Most studies evaluating the cancer-detection capability of DCBE utilized a methodology in which all patients in an institution- or population-based database that had been diagnosed with CRC were assessed for a history of a prior DCBE within a defined time frame, the length of which was not consistent between studies but usually ranged from 2 to 5 years. The assumption was that missed cancers on DCBE would subsequently be clinically detected. The majority of these studies showed sensitivity for cancer of 85% to 97% (137–150).
Review of the literature concerning the performance of DCBE for polyps is more difficult due to the described biases and heterogeneity of study design; in particular, the target lesion and thresholds considered clinically significant often varied based upon size and/or morphology. Two studies involving truly asymptomatic individuals were performed in surveillance groups with a history of prior adenoma removal (151,152). These demonstrated sensitivities of 48% (N = 23) for adenomas ≥1 cm and 73% (N = 56) for adenomas > 7 mm, respectively. It should be noted that in the former study, the DCBE detected 75% (6 of 8) with advanced histology (153).
DCBE—Benefits, Limitations, and Harms. The potential benefits derived from the DCBE are that it evaluates the entire colon in almost all cases and can detect most cancers and the majority of significant polyps. DCBE also provides an opportunity for a full structural examination for individuals for whom colonoscopy has either failed or is contraindicated.
DCBE has several limitations. The acceptability of DCBE may be limited by the requirement for extensive colonic preparation, and some patients experience discomfort during and after the procedure. Suboptimal preparation can reduce both sensitivity and specificity. Further, there is no opportunity for biopsy or polypectomy, and any individual with findings of polyps ≥6 mm on DCBE should undergo colonoscopy. The lower sensitivity for significant adenomas when compared with colonoscopy may result in less favorable outcomes regarding morbidity and mortality from CRC. DCBE is also limited by the operator dependence of the radiologist or technologist performing the examination, as well as by the radiologist interpreting the examination. DCBE is a relatively safe procedure with a lower perforation rate when compared with colonoscopy (1 of 25,000 versus 1 of 1,000 to 2,000) (154).
Quality Assurance. The DCBE is a full structural examination of the entire colon that can be performed by radiologists or radiology residents and trained technicians under the supervision of a radiologist. Factors that can affect the quality of the DCBE examination include 1) ability to fully evaluate the entire colon due to lack of retained barium or collapse of segments of the colon; 2) adequacy of the bowel preparation; 3) patient’s ability to stand and be imaged in prone and supine positions; and 4) reader’s experience in interpretation. Caution is advised when performing a DCBE on the same day after polypectomy to avoid a perforation. The ACR has published guidelines that detail the basic requisites for a high-quality examination (155), as well as a quality-assurance manual for the DCBE (155). Interaction with referring physicians to correlate radiologic findings with endoscopic and/or surgical outcomes may also be an effective ongoing quality assurance in clinical practice.
DCBE—Other Issues. It is likely that the decline in the use of DCBE for CRC screening in average-risk adults will continue (156–158). This decline in the utilization of DCBE has had an impact on training programs, as radiology residents have had less opportunity to develop the necessary skills to perform the procedure properly. Moreover, although there likely are sufficient numbers of radiologists in clinical practice who are available currently to perform DCBE studies, there has been a decline in radiologists’ enthusiasm for the DCBE due to its labor-intensive nature, the low reimbursement rate, and greater interest in newer and more complex technologies such as computed tomography (CT) and magnetic resonance imaging (MRI). Based on these trends, it is likely that in the next 5 years, that there will be even fewer radiologists adequately trained to perform this procedure due to the low volume of DCBE studies currently being requested, as well as low professional interest. At present, the DCBE remains an option for direct imaging of the entire colon and may be of particular value where colonoscopy resources are limited or colonoscopy is contraindicated or less likely to be successful (eg, prior incomplete colonoscopy, prior pelvic surgery, etc.).
DCBE—Conclusions and Recommendations. DCBE every 5 years is an acceptable option for CRC screening in average-risk adults aged 50 years and older. Discussions with patients should include a description of the test characteristics, the importance of adherence to a thorough colon cleansing, test accuracy, the likelihood of a positive test, and the need for subsequent colonoscopy if the test is abnormal. The choice of DCBE for screening can be made on an individual basis, depending on factors such as personal preference, cost, and the local availability of trained radiologists able to offer a high-quality examination.
CTC, also referred to as virtual colonoscopy, is a minimally invasive imaging examination of the entire colon and rectum. CTC uses CT to acquire images and advanced 2-dimensional (2D)- and 3-dimensional (3D)-image display techniques for interpretation. Since its introduction in the mid-1990s, there have been rapid advancements in CTC technology. Multidetector CT now permits image acquisition of thin 1 to 2 mm slices of the entire large intestine well within breath-hold imaging times. Computer imaging graphics allow for visualization of 3D endoscopic flight paths through the inside of the colon, which are simultaneously viewed with interactive 2D images. The integrated use of the 3D and 2D techniques allows for ease of polyp detection, as well as characterization of lesion density and location. The 2D images also allow for limited evaluation of the extracolonic structures.
Adequate bowel preparation and gaseous distention of the colon are essential to ensure a successful examination. Patients typically undergo full cathartic preparation along with a clear liquid diet the day before the study, similar to the requirements for colonoscopy. Tagging of residual solid stool and fluid with barium and/or iodine oral contrast agents is being increasingly used and validated in large trials. At CT, a small caliber rectal catheter is inserted into the rectum, followed by automated or manual insufflation of room air or carbon dioxide. Intravenous contrast generally is not given to patients undergoing screening but can be helpful in some patients with more advanced symptoms. Typically, the entire procedure on the CT table takes approximately 10 minutes, with no sedation or recovery time needed. Research into noncathartic approaches to minimize the bowel preparation is underway, but this technique has not yet been validated in a multicenter screening trial (159–161). However, under conditions where same-day or next-day referral for colonoscopy would be possible, one drawback of noncathartic CTC is that a cathartic bowel preparation would still be required prior to removal of polyps.
CTC—Efficacy and Test Performance. No prospective, randomized, controlled clinical trial has been initiated (nor is one planned) to directly demonstrate the efficacy of CTC in reducing mortality from CRC. Given the cumulative body of evidence in support of CRC screening for reducing mortality and the value of polypectomy in reducing incidence, studies of CTC have focused on the detection of advanced neoplasia.
The test performance characteristics of CTC for polyp detection are derived by using optical colonoscopy (OC) as the reference standard. Early single-center CTC clinical trials involving small, polyp-rich cohorts (162–164) provided encouraging initial results and served as proof of concept that paved the way for larger multicenter screening trials. Two early trials by Cotton et al (165) and Rocky et al (166) included approximately 600 subjects each and observed per-patient sensitivity for large polyps of 55% and 59%, respectively. However, these 2 studies did not evaluate screening in an asymptomatic population, nor did they apply the latest CTC techniques. A more recently initiated multi-institutional screening trial using more advanced CTC techniques demonstrated more favorable performance. Pickhardt et al studied 1,233 asymptomatic adults and introduced the techniques of stool tagging and primary 3D polyp detection, neither of which were used in the 2 earlier multi-institutional trials (167). This trial reported a 94% sensitivity for large adenomas, with a per-patient sensitivity for adenomas ≥6 mm of 89%. In 2005, 2 meta-analyses reviewed the cumulative published CTC performance data, including both high-risk and screening cohorts, with one analysis representing 33 studies on 6,393 patients (168,169). On a per-patient basis, pooled CTC sensitivity and specificity for large (≥10 mm) polyps was found to be 85% to 93% and 97%, respectively. Pooled sensitivity and specificity for detection of small polyps (6 to 9 mm) was 70% to 86% and 86% to 93%, respectively. Of note, the pooled CTC sensitivity for invasive CRC was 96% (168), comparable with the reported sensitivity for OC (119,121).
There also are a number of CTC trials currently in progress within the United States and Europe. Initial results from smaller screening trials utilizing 3D polyp detection by Cash et al (170) and Graser et al (171) have shown CTC performance characteristics similar to that of Pickhardt et al, providing at least a measure of independent validation for this screening technique. Also of particular interest is the recently completed ACRIN Study 6664: National CT Colonography Trial, which is sponsored and funded by the National Cancer Institute. The primary aim of this trial was to assess CTC performance for large adenomas and advanced neoplasia in a large screening cohort of 2,500 patients across 15 institutions. State-of-the-art techniques included oral contrast tagging, colonic distention with automated carbon dioxide delivery, multidetector row CT (≥16 slice) with thin collimation, and both 2D and 3D polyp detection on dedicated CTC software systems. Specialized training and achievement of a high level of expertise were required of the radiologists prior to participation in the study. Preliminary findings announced at the 2007 annual meeting of ACRIN on September 28, 2007,were consistent with other recent studies using state-of-the-art techniques.
Beyond validation, a recent study demonstrated the efficacy of CTC to select patients who would benefit from therapeutic polypectomy. Kim et al recently reported comparative results from primary CTC (with selective recommendation for therapeutic colonoscopy) and primary OC screening arms among 3,120 and 3,163 mostly asymptomatic adults, respectively (172). Although this study did not randomize participants to CTC versus OC, apart from a slightly higher proportion of individuals with a family history in the OC group, the 2 groups were similar. Similar rates of advanced neoplasia were found in each group, with 3.2% in the CTC group and 3.4% in the OC group (172).
CTC—Benefits, Limitations, and Harms. CTC provides a time-efficient procedure with good accuracy and minimal invasiveness. No sedation or recovery time is required, nor is a chaperone needed to provide transportation after the procedure. Time permitting, patients can return to work on the same day. However, some limitations to CTC exist, ranging from access issues to potential harms from the examination. Because CTC is relatively early in its utilization, there are fewer data relative to other CRC screening tests for evaluating benefits, limitations, and harms. Thus, continued development of best practice standards is a high priority, as is monitoring the performance of CTC as access and utilization increases. At this time, reimbursement for screening CTC is very limited, although 47 states now offer Medicare reimbursement for diagnostic CTC where the clinical indication is limited to incomplete OC (173). However, because reimbursement for screening still is uncommon, the current professional capacity to deliver CTC also is limited, although capacity is expected to increase when third-party payers begin providing reimbursement
CTC requires the same full cathartic bowel preparation and restricted diet as colonoscopy, which may decrease patient adherence. As an "imaging-only," nontherapeutic evaluation of the colon, patients with polyps of significant size will require therapeutic colonoscopy for subsequent polypectomy. Thus, it is possible to offer same-day polypectomy to patients for whom colonoscopy is recommended without the need for additional bowel preparation, although this convenience for patients requires coordination between radiology and gastroenterology departments (174). Where such coordination does not exist, patients will need to undergo an additional bowel preparation. While older oral tagging protocols would have precluded same-day colonoscopy, revised, more efficient tagging protocols have successfully allowed therapeutic colonoscopy on the same day.
CTC is similar to endoscopy and DCBE with respect to the quality of interpretation being highly operator dependent, and thus initiatives towards training and certification are important. Detection of flat lesions has been variable, ranging from sensitivities of 13% to 65% in early CTC studies 175 to 80% when using multidetector CT and combined 3D-2D polyp detection (176). However, debate continues over the prevalence and significance of flat colorectal lesions (177–179).
The accuracy of CTC is influenced by lesion size, and the sensitivity and specificity of CTC improves with polyp size. The accuracy of CTC in measuring polyp size is of particular importance since accurate size estimation is critical for appropriate patient management and for minimizing the false-positive rate. While earlier studies using rudimentary software applied to wide-slice thicknesses and 2D images showed poor concordance with prefixation polyp size (180), modern CT technology producing 3D images results in more accurate size estimates (181–183). The ability to ensure consistent polyp size measurements during examinations is a high priority for quality-assurance initiatives since it will influence referrals for polypectomy. Pickhardt et al showed that specificity (when polyps were matched for size) was 97.4% for lesions ≥1 cm but declines to 84.5% for all lesions to all lesions ≥6mm (167). The incremental increase in the false-positive rate associated with polyps between 6 to 8 mm could add significantly to the cost of screening, and thus it will be important to monitor sensitivity and specificity in the clinical setting and identify strategies to improve specificity without diminishing sensitivity.
There is controversy over the long-term potential harms associated with radiation dose effects from CT examinations. One aspect of this controversy relates to risk-estimation models, and the other pertains to the long-term risk of cancer from single and repeated medical imaging exposures (184, 185). While current estimates of the potential cancer risk related to low-dose radiation exposures during medical procedures derive from linear nonthreshold models based on long-term outcomes in survivors of acute radiation doses from atomic weapons, there is disagreement over whether this model truly is applicable to periodic exposures from medical imaging (186). In a recent position statement issued by the Health Physics Society, the health effects of low-dose radiation exposure (defined as below 50 to 100 mSv—a threshold many times higher than typical CTC levels) were considered to be "either too small to be observed or are nonexistent." (187) Nevertheless, although this risk may be theoretical, there is a growing concern that more individuals are receiving multiple diagnostic evaluations with ionizing radiation over a lifetime and that for some individuals the doses over a lifetime can reach levels that are sufficiently high to be of concern. It is important to put these issues into
Using the linear, no-threshold radiation-risk estimate, a CTC examination in a 50-year-old individual with an estimated organ dose to the colon of 7 to 13 mSv (65 mAs) is estimated to add an additional 0.044% to the lifetime risk of colon cancer (188). Because organ radiosensitivity declines with increasing age, this organ dose is halved for the same examination taking place at age 70 years. In this same evaluation, the additional lifetime risk of cancer in any site associated with a single CTC examination at age 50 years was 0.14%, although the authors stated with optimized techniques this risk could be reduced by a factor of 5- to10-fold. More efficient dose protocols using 50 mAs on 4DCT, similar to the ACR-defined protocols, have demonstrated decreased estimated organ dose ranges of 5 to 8 mSv (189). While acknowledging there is uncertainty about potential harms from single or multiple CTC screening examinations, current ACR quality metrics for CTC define low-dose parameters as a best practice for minimizing risk to patients (190).
Since CTC is a minimally invasive test, the risk for colonic perforation during screening is extremely low. In the collective experience of the International Working Group on Virtual Colonoscopy, there were no cases of perforation in over 11,000 screening CTC examinations, and out of nearly 22,000 total CTC examinations (screening and diagnostic), there was only one symptomatic perforation, corresponding to a symptomatic perforation rate of 0.005% (191). Some studies of symptomatic patients, however, have reported higher perforation rates, ranging from 0.03% (1 in 3,400 patients) to 0.06% (1 in 1,700 patients). (192,193) Colonic distention with low-pressure carbon dioxide delivery may be safer than insufflation of room air (191). Rates of perforation are part of the quality metrics being
Because CTC produces an image not only of the colon but also the upper and lower abdomen, there is a chance that incidental extracolonic findings will be observed. Although the overall rates of extracolonic findings have been reported to range from 15% to 69%, the incidence of clinically significant extracolonic findings at CTC has ranged from 4.5% to 11% in various patient cohorts (194–197). In an asymptomatic screening population, the incidence of unsuspected but potentially important extracolonic findings is approximately 4.5%, but findings of minimal or moderate potential clinical significance, such as cholelithiasis (6%) and nephrolithiasis (8%), are more common (197). While there are potential benefits from serendipitous findings, there also are associated risks and costs that need to be considered when these findings are false positives. These include further radiologic imaging and, thus, added organ dose, potential for adverse outcomes associated with tissue sampling for abnormalities that are not resolved with additional imaging, as well as the direct and indirect costs to the patient. The implementation of structured reporting of extracolonic findings and monitoring trends in subsequent diagnostic workups and adherence with quality metrics are being evaluated through the National Radiology Data Registry (NRDR), the ACR’s national data warehouse.
Quality Assurance. Similar to the call to action for measuring quality of colonoscopy (198), the implementation of CTC will require quality metrics to be defined and implemented in clinical practice. Quality of CTC examinations will depend on 1) proper bowel preparation; 2) adequate insufflation of the colon and appropriate use of CTC technique parameters at image acquisition; 3) adequate training of the interpreting physician in the use of 2D- and 3D-image display techniques; and 4) documentation of clinically significant colonic and extracolonic lesions to referring physicians. In 2005, the ACR Practice Guidelines for the Performance of Computed Tomography (CT) Colonography in Adults was published, encompassing the techniques, quality control, clinical uses, training, and communication of results for CTC (190). An update of these guidelines is planned following publication of the results of the ACRIN CTC screening trial. In 2006, the ACR Colon Cancer Committee outlined practice-based quality metrics for CTC, encompassing process measures of CTC technique and image quality; patient preparation; and outcomes measures such as rates of true positives, colonic perforation, and incidence of extracolonic findings. These quality metrics are to begin a pilot phase in late 2007, with data entry in the NRDR database. The ACR has begun construction of an interactive, hands-on training facility for CTC and will begin training courses in early 2008. A process for individual certification and proficiency is being evaluated.
CTC—Other Issues. Standardization of the evolving technology and consensus related to the reporting of findings will be essential for effective implementation of CTC screening. A consensus statement of a standardized reporting structure for CTC findings was recently published, modeled after the Breast Imaging Reporting and Data System’s (BI-RADS) reporting of mammography (199). This reporting structure, termed the "CT Colonography Reporting and Data System (C-RADS)," describes how to report lesion size, morphology, and location, with a summary category score per patient.
The management of CTC findings is an important part of a CTC screening program. At this time, there is consensus that all patients with one or more polyps ≥10 mm or 3 or more polyps ≥6 mm should be referred for colonoscopy (200). The management of patients with fewer polyps (>3) in which the largest polyp is 6 to 9 mm remains controversial. Such polyps are routinely removed if found at OC because of the opportunity and the risk, albeit low, of advanced neoplasia. However, in studies that have been limited to screening cohorts, among individuals whose largest polyp is 6 to 9 mm in size, the prevalence of advanced features tends to be low (3.4% to 6.6%) (201,202). At this time, there is ongoing research using CTC surveillance to evaluate the natural history of polyps in this size range. Based on expert consensus and until further evidence is available to provide additional guidance, a reasonable approach at this time for patients with 6 to 9 mm polyps identified on CTC is to recommend therapeutic colonoscopy. Patients who decline referral to colonoscopy or who are not good candidates for colonoscopy should be offered surveillance with CTC.
Optimal management of patients whose largest polyp is <6 mm detected on CTC is uncertain. Experts from the American Gastroenterological Association, the American College of Gastroenterology, and the ACR have reported a range of policies on how to handle these lesions (190,203,204). There is general agreement that the risk of advanced features in patients whose largest polyp is ≤5 mm is very low. In a recent study that is able to provide this estimate in a screening cohort, the prevalence of advanced neoplasia in patients whose largest polyp was ≤5 mm was 1.7% (D.A.L, personal communication, December 14, 2007) (202). At this time, there is a pressing need for multidisciplinary consensus on the reporting and clinical management of patients whose largest polyp is <6 mm.
CTC—Conclusions and Recommendations. In terms of detection of colon cancer and advanced neoplasia, which is the primary goal of screening for CRC and adenomatous polyps, recent data suggest CTC is comparable to OC for the detection of cancer and polyps of significant size when state-of-the-art techniques are applied. In previous assessments of the performance of CTC, the ACS concluded that data were insufficient to recommend screening with CTC for average-risk individuals (19). Based on the accumulation of evidence since that time, the expert panel concludes that there are sufficient data to include CTC as an acceptable option for CRC screening.
Screening of average-risk adults with CTC should commence at age 50 years. The interval for repeat exams after a negative CTC has not been studied, and is uncertain. However, if current studies confirm the previously reported high sensitivity for detection of cancer and of polyps ≥6 mm, it would be reasonable to repeat exams every 5 years if the initial CTC is negative for significant polyps until further studies are completed and are able to provide additional guidance. Until there is more research on the safety of observation, colonoscopy should be offered to patients whose largest polyp is 6 mm or greater. CTC surveillance could be offered to those patients who would benefit from screening but either decline colonoscopy or who are not good candidates for colonoscopy for one or more reasons. However, if colonoscopy is contraindicated because the patient is not likely to benefit from screening due to life-limiting comorbidity, then neither CTC nor any other CRC screening test would be appropriate.
There is compelling evidence to support screening average-risk individuals over age 50 years to detect and prevent CRC. Screening of average-risk individuals can reduce CRC mortality by detecting cancer at an early, curable stage and by detecting and removing clinically significant adenomas. No CRC screening test is perfect, either for cancer detection or adenoma detection. Each test has unique advantages, each has been shown to be cost-effective (205–208), and each has associated limitations and risks. Patient preferences and availability of resources play an important role in the selection of screening tests. In this update of the guidelines for CRC screening, we have placed an emphasis on the value of preventing CRC, sought to address the importance of test sensitivity in the presence of low rates of programmatic screening, and attempted to provide improved guidance about test characteristics and quality issues to referring clinicians. Ideally, screening should be supported in a programmatic fashion that begins with risk stratification and the results from an initial test and continues through proper follow up based on findings. The effectiveness of any single test or combination of tests depends on high rates of programmatic adherence and quality. Based on differing incidence rates and observations of different patterns of polyp and cancer distribution in certain subsets of patients (ie, the elderly, women, and ethnic minorities, etc.), some experts have suggested that these groups may require different screening recommendations (209,210). The expert panel reviewed and discussed the evidence and rationale for and against including different screening recommendations in this update for various demographic subgroups that have been shown to be at somewhat higher or lower than average risk for disease or proximal lesions. After some consideration, this issue was postponed for further consideration at a later time for a number of reasons, although principally because 1) there are no current data to indicate that CRC incidence and mortality in these groups would be positively impacted by tailored screening recommendations; and 2) screening rates among all groups remain low under existing guidelines and providing different (and, in some cases, more limited) screening options has the potential to increase confusion, complexity, and workload, and thus might add additional barriers to screening that would affect all groups. This is an area of research that the collaborating organizations will continue to monitor closely.
In this update of the CRC screening guidelines, we have focused on screening in average-risk adults and have not reviewed recent literature on CRC screening or surveillance for individuals at increased and high risk. Individuals at increased risk due to a history of adenomatous polyps; a personal history of curative-intent resection of CRC; a family history of either CRC or colorectal adenomas diagnosed in a first-degree relative before age 60 years; or high risk due to a history of inflammatory bowel disease of significant duration or the presence of one of 2 hereditary syndromes should continue to follow recommendations issued previously by the ACS or USMSTF (18,24). These recommendations are summarized in Table 3.
|Risk Category||Age to Begin||Recommendation||Comment|
|Increased Risk—Patients with History of Polyps at Prior Colonoscopy|
|Patients with small rectal hyperplastic polyps (26)||—||Colonoscopy or other screening options at intervals recommended for average-risk individuals||An exception is patients with a hyperplastic polyposis syndrome. They are at increased risk for adenomas and colorectal cancer and need to be identified for more intensive follow up.|
|Patients with 1 or 2 small tubular adenomas with low-grade dysplasia (26)||5 to 10 years after the initial polypectomy||Colonoscopy||The precise timing within this interval should be based on other clinical factors (such as prior colonoscopy findings, family history, and the preferences of the patient and judgment of the physician).|
|Patients with 3 to 10 adenomas or 1 adenoma >1 cm or any adenoma with villous features or high-grade dysplasia (26)||3 years after the initial polypectomy||Colonoscopy||Adenomas must have been completely removed. If the follow-up colonoscopy is normal or shows only 1 or 2 small, tubular adenomas with low-grade dysplasia, then the interval for the subsequent examination should be 5 years.|
|Patients with >10 adenomas on a single examination (26)||<3 years after the initial polypectomy||Colonoscopy||Consider the possibility of an underlying familial syndrome.|
|Patients with sessile adenomas that are removed piecemeal (26)||2 to 6 months to verify complete removal||Colonoscopy||Once complete removal has been established, subsequent surveillance needs to be individualized based on the endoscopist’s judgment. Completeness of removal should be based on both endoscopic and pathologic assessments.|
|Increased Risk—Patients with Colorectal Cancer|
|Patients with colon and rectal cancer should undergo high-quality perioperative clearing (25)||3 to 6 months after cancer resection, if no unresectable metastases are found during surgery; alternatively, colonoscopy can be performed intraoperatively||Colonoscopy||In the case of nonobstructing tumors, this can be done by preoperative colonoscopy. In the case of obstructing colon cancers, CTC with intravenous contrast or DCBE can be used to detect neoplasms in the proximal colon.|
|Patients undergoing curative resection for colon or rectal cancer (2)||1 year after the resection (or 1 year following the performance of the colonoscopy that was performed to clear the colon of synchronous disease)||Colonoscopy||This colonoscopy at 1 year is in addition to the perioperative colonoscopy for synchronous tumors. If the examination performed at 1 year is normal, then the interval before the next subsequent examination should be 3 years. If that colonoscopy is normal, then the interval before the next subsequent examination should be 5 years. Following the examination at 1 year, the intervals before subsequent examinations may be shortened if there is evidence of HNPCC or if adenoma findings warrant earlier colonoscopy. Periodic examination of the rectum for the purpose of identifying local recurrence, usually performed at 3- to 6-month intervals for the first 2 or 3 years, may be considered after low-anterior resection of rectal cancer.|
|Increased Risk—Patients with a Family History|
|Either colorectal cancer or adenomatous polyps in a first-degree relative before age 60 years or in 2 or more first-degree relatives at any age (24)||Age 40 years or 10 years before the youngest case in the immediate family||Colonoscopy||Every 5 years|
|Either colorectal cancer or adenomatous polyps in a first-degree relative ≥age 60 years or in 2 second-degree relatives with colorectal cancer (24)||Age 40 years||Screening options at intervals recommended for average-risk individuals||Screening should begin at an earlier age, but individuals may choose to be screened with any recommended form of testing.|
|Genetic diagnosis of FAP or suspected FAP without genetic testing evidence (24)||Aged 10 to 12 years||Annual FSIG to determine if the individual is expressing the genetic abnormality and counseling to consider genetic testing.||If the genetic test is positive, colectomy should be considered.|
|Genetic or clinical diagnosis of HNPCC or individuals at increased risk of HNPCC (24)||Aged 20 to 25 years or 10 years before the youngest case in the immediate family||Colonoscopy every 1 to 2 years and counseling to consider genetic testing||Genetic testing for HNPCC should be offered to first-degree relatives of persons with a known inherited MMR gene mutation. It should also be offered when the family mutation is not already known, but 1 of the first 3 of the modified Bethesda Criteria is present.|
|Inflammatory bowel disease, (24) chronic ulcerative colitis, and Crohn’s colitis||Cancer risk begins to be significant 8 years after the onset of pancolitis or 12 to 15 years after the onset of left-sided colitis||Colonoscopy with biopsies for dysplasia||Every 1 to 2 years; these patients are best referred to a center with experience in the surveillance and management of inflammatory bowel disease|
|Abbreviations: FSIG, flexible sigmoidoscopy; DCBE, double-contrast barium enema; CTC, computed tomographic colonography; FAP, familial adenomatous polyposis; HNPCC, hereditary nonpolyposis colon cancer; MMR, mismatch repair.|
There appears to be a clear need for institutionally based quality-assurance programs to improve the quality of CRC screening. This guideline update emphasizes issues for quality assurance across colorectal screening modalities, spanning training requirements, optimal techniques to complete examination, screening intervals, and appropriate recommendations for follow up. In contrast, cost-effectiveness is not specifically discussed in this document, based on the numerous complexities of adequately addressing this topic, including understanding real costs in different environments, differences in test performance and interpretation, and wide variability of screening intervals in different settings. It is hoped that compliance with improvements in quality assurance will both improve quality and promote cost-effectiveness. Clearly, better definition of the target lesion of clinical importance is needed across modalities. As new technologies evolve that detect but do not remove polyps, multidisciplinary consensus is needed to best manage a patient programmatically for follow-up polypectomy versus surveillance intervals. Although there are some ongoing studies of the natural history of small polyps, evidence-based data will probably take 10 to 20 years to meaningfully translate into clinical-practice recommendations. In this interim, the current recommendations try to address these issues with expert consensus based on existing data. Multidisciplinary groups, such as the National Colorectal Cancer Roundtable, may be able to serve as an effective forum for the development of a consensus across specialties
In conclusion, it is our hope that these new recommendations will facilitate increased rates of CRC screening and that referring clinicians find these new guidelines ease some of the challenges they have experienced in promoting CRC screening to their patients.
We wish to acknowledge the role of the advisory committees, external experts, and helpful comments and recommendations from reviewers and organizational representatives who reviewed various drafts of this document.
Conflict of Interest
Disclosures: Workgroup members were asked to disclose relationships, including potential financial conflicts of interest. The following was disclosed: D. Lieberman served on the scientific advisory board for Exact Sciences, ending September 30, 2007. B. McFarland receives honoraria for serving on the medical advisory boards for Vital Images and Medicsight. P. Pickhardt serves as a paid consultant to Covidien, Viatronix, Fleet, Medicsight, and Philips. D. Rex receives an honorarium for serving as a speaker and research support for serving as an investigator for Olympus; serves on the scientific advisory board and receives research support for serving as an investigator for Given Imaging; and serves on the scientific advisory boards for Avantis, NeoGuide, G.I. View, and American BioOptics. M. Rodriguez-Bigas is on the speakers bureau of Genzyme. R.Wender serves on the scientific advisory boards for Epigenomics, GeneNews, and G.I. View, but receives no personal income for doing so. D. Johnson serves as an Exact Sciences clinical investigator, but has received no support since 2006. C. D. Johnson and Mayo Clinic have licensed a CT colonography software patent to GE Medical Systems.
ACS Colorectal Cancer Advisory Group
Members: Bernard Levin, MD (Chair); Professor Emeritus, The University of Texas MD Anderson Cancer Center, Houston, TX; Tim Byers, MD, MPH (Vice Chair); Professor, Preventive Medicine and Biometrics, University of Colorado Health Sciences Center, Denver, CO; Briggs W. Andrews, Esq.; Senior Vice President; and General Counsel of Carilion Health System, Roanoke, VA; Elvan Daniels, MD; Associate Director for Community-Oriented Primary Care, Morehouse School of Medicine, Atlanta, GA; Lovell Jones, PhD; Professor of Gynecologic Oncology; and Director, Center of Excellence for Research on Minority Health, The University of Texas MD Anderson Cancer Center, Houston, TX; Gordon Klatt, MD; Medical Director, Cancer Programs, Mt. Rainier Surgical Association, Tacoma, WA; Theodore R. Levin, MD; Staff Physician, Gastroenterology Department, Kaiser Permanente Walnut Creek Medical Center,Walnut Creek, CA; Pamela McAllister, PhD; Founding Member and Research Advocate, Colorectal Cancer Coalition, Alexandria, VA; Beth McFarland, MD; Adjunct Professor, Mallinckrodt Institute of Radiology, St. Luke’s Hospital, Diagnostic Imaging Associates, Chesterfield, MO; Sigurd Normann, MD, PhD; Professor, Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL; Miguel Rodriguez-Bigas, MD; Professor of Surgery, Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX; Alan Thorson, MD; Associate Professor of Surgery; and Program Director, Section of Colon and Rectal Surgery, Creighton University School of Medicine; and Clinical Associate Professor of Surgery, University of Nebraska College of Medicine, Omaha, NE; Richard Wender, MD; Chair; and Alumni Professor, Department of Family and Community Medicine, Thomas Jefferson University, Philadelphia, PA
ACS Staff and Consultants: Durado Brooks, MD, MPH; Director, Prostate and CRC, Cancer Control Science Department, American Cancer Society, Atlanta, GA; Robert A. Smith, PhD; Director, Cancer Screening, Cancer Control Science Department, American Cancer Society, Atlanta, GA; Kimberly S. Andrews; Research Associate, Cancer Control Science Department, American Cancer Society, Atlanta, GA; Chiranjeev Dash, MD, MPH; Doctoral Candidate, Department of Epidemiology, Emory University, Rollins School of Public Health, Atlanta, GA
US Multi-Society Task Force
Members: David A. Lieberman, MD; Chief, Division of Gastroenterology, Oregon Health and Science University, Portland Veterans Medical Center, Portland, OR; John Bond, MD; Chief, Gastroenterology Section, Minneapolis Veterans Affairs Medical Center, Minneapolis, MN; Francis M. Giardiello, MD; John G. Rangos Sr. Professor of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD; David Johnson, MD; Professor of Medicine, Eastern Virginia Medical School, Norfolk, VA; Theodore R. Levin, MD; Staff Physician, Gastroenterology Department, Kaiser Permanente Walnut Creek Medical Center, Walnut Creek, CA; Douglas K. Rex, MD; Professor of Medicine, Indiana University, Indianapolis, IN; Clyde Watkins, Jr., MD; Physician, Capstone Medical Group PC, Lithonia, GA; Sidney J. Winawer, MD; Attending Physician, Memorial Sloan-Kettering Cancer Center, New York, NY
American College of Radiology Colon Cancer Committee
Members: Beth McFarland, MD; Adjunct Professor of Radiology, Washington University; St. Luke’s Hospital and Center for Diagnostic Imaging, Chesterfield, MO; Seth Glick, MD; Clinical Professor of Radiology, University of Pennsylvania Health System, Philadelphia, PA; Perry Pickhardt, MD; Associate Professor, Radiology Department, University of Wisconsin Hospital and Clinics, Madison, WI; C. Daniel Johnson, MD; Chairman and Professor of Radiology, Radiology Department, Mayo Clinic, Scottsdale, AZ
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